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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

Item Type:Article
Title:Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
Creators Name:Meyer, M. and de Angelis, M.H. and Wurst, W. and Kuehn, R.
Abstract:Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.
Keywords:Bacterial Proteins, Base Sequence, DNA, Embryonic Stem Cells, Endonucleases, Gene Targeting, Genetic Recombination, Genetic Vectors, Luminescent Proteins, Molecular Sequence Data, Pregnancy, Proteins, Reporter Genes, Untranslated RNA, Zinc Fingers, Zygote, Animals, Mice
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:107
Number:34
Page Range:15022-15026
Date:24 August 2010
Official Publication:https://doi.org/10.1073/pnas.1009424107
PubMed:View item in PubMed

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