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Modeling disease mutations by gene targeting in one-cell mouse embryos

Item Type:Article
Title:Modeling disease mutations by gene targeting in one-cell mouse embryos
Creators Name:Meyer, M. and Ortiz, O. and Hrabe de Angelis, M. and Wurst, W. and Kuehn, R.
Abstract:Gene targeting by zinc-finger nucleases in one-cell embryos provides an expedite mutagenesis approach in mice, rats, and rabbits. This technology has been recently used to create knockout and knockin mutants through the deletion or insertion of nucleotides. Here we apply zinc-finger nucleases in one-cell mouse embryos to generate disease-related mutants harboring single nucleotide or codon replacements. Using a gene-targeting vector or a synthetic oligodesoxynucleotide as template for homologous recombination, we introduced missense and silent mutations into the Rab38 gene, encoding a small GTPase that regulates intracellular vesicle trafficking. These results demonstrate the feasibility of seamless gene editing in one-cell embryos to create genetic disease models and establish synthetic oligodesoxynucleotides as a simplified mutagenesis tool.
Keywords:Base Sequence, Gene Targeting, Genetic Predisposition to Disease, Genetic Vectors, Mammalian Embryo, Molecular Sequence Data, Mutation, Nucleic Acid Sequence Homology, Animals, Mice
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:109
Number:24
Page Range:9354-9359
Date:12 June 2012
Official Publication:https://doi.org/10.1073/pnas.1121203109
PubMed:View item in PubMed

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