Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

IPG strip-based peptide fractionation for shotgun proteomics

Item Type:Article
Title:IPG strip-based peptide fractionation for shotgun proteomics
Creators Name:Eravci, M. and Sommer, C. and Selbach, M.
Abstract:Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible.
Keywords:Shotgun Proteomics, Peptide Fractionation, Isoelectric Focusing (IEF), Immobilized pH Gradient (IPG)
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Shotgun proteomics : methods and protocols
ISSN:1064-3745
ISBN:978-1-4939-0684-0
Publisher:Springer / Humana Press (U.S.A.)
Volume:1156
Page Range:67-77
Number of Pages:492
Date:2014
Official Publication:https://doi.org/10.1007/978-1-4939-0685-7_5
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library