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| Item Type: | Article |
|---|---|
| Title: | IPG strip-based peptide fractionation for shotgun proteomics |
| Creators Name: | Eravci, M. and Sommer, C. and Selbach, M. |
| Abstract: | Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible. |
| Keywords: | Shotgun Proteomics, Peptide Fractionation, Isoelectric Focusing (IEF), Immobilized pH Gradient (IPG) |
| Source: | Methods in Molecular Biology |
| Series Name: | Methods in Molecular Biology |
| Title of Book: | Shotgun proteomics : methods and protocols |
| ISSN: | 1064-3745 |
| ISBN: | 978-1-4939-0684-0 |
| Publisher: | Springer / Humana Press |
| Volume: | 1156 |
| Page Range: | 67-77 |
| Number of Pages: | 492 |
| Date: | 2014 |
| Official Publication: | https://doi.org/10.1007/978-1-4939-0685-7_5 |
| PubMed: | View item in PubMed |
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