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Identification of small ORFs in vertebrates using ribosome footprinting and evolutionary conservation

Official URL:https://doi.org/10.1002/embj.201488411
PubMed:View item in PubMed
Creators Name:Bazzini, A.A. and Johnstone, T.G. and Christiano, R. and Mackowiak, S.D. and Obermayer, B. and Fleming, E.S. and Vejnar, C.E. and Lee, M.T. and Rajewsky, N. and Walther, T.C. and Giraldez, A.J.
Journal Title:EMBO Journal
Journal Abbreviation:EMBO J
Volume:33
Number:9
Page Range:981-993
Date:2 May 2014
Keywords:Micropeptides, Non-Coding RNA, Ribosome Profiling, Small ORFs, Translation, Animals, Zebrafish
Abstract:Identification of the coding elements in the genome is a fundamental step to understanding the building blocks of living systems. Short peptides (< 100 aa) have emerged as important regulators of development and physiology, but their identification has been limited by their size. We have leveraged the periodicity of ribosome movement on the mRNA to define actively translated ORFs by ribosome footprinting. This approach identifies several hundred translated small ORFs in zebrafish and human. Computational prediction of small ORFs from codon conservation patterns corroborates and extends these findings and identifies conserved sequences in zebrafish and human, suggesting functional peptide products (micropeptides). These results identify micropeptide-encoding genes in vertebrates, providing an entry point to define their function in vivo.
ISSN:0261-4189
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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