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| Item Type: | Article |
|---|---|
| Title: | Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein |
| Creators Name: | Olal, D. and Dick, A. and Woods, V.L. and Liu, T. and Li, S. and Devignot, S. and Weber, F. and Saphire, E.O. and Daumke, O. |
| Abstract: | Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses. |
| Keywords: | Binding Sites, Molecular Models, Nucleocapsid Proteins, Protein Binding, Protein Multimerization, Quaternary Protein Structure, Sandfly fever Naples virus, Secondary Protein Structure, Viral RNA, Virus Assembly, X-Ray Crystallography |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 42 |
| Number: | 9 |
| Page Range: | 6025-6037 |
| Date: | 14 May 2014 |
| Official Publication: | https://doi.org/10.1093/nar/gku229 |
| PubMed: | View item in PubMed |
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