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| Item Type: | Article |
|---|---|
| Title: | Plasma membrane lipid-protein interactions affect signaling processes in sterol-biosynthesis mutants in Arabidopsis thaliana |
| Creators Name: | Zauber, H. and Burgos, A. and Garapati, P. and Schulze, W.X. |
| Abstract: | The plasma membrane is an important organelle providing structure, signaling and transport as major biological functions. Being composed of lipids and proteins with different physicochemical properties, the biological functions of membranes depend on specific protein-protein and protein-lipid interactions. Interactions of proteins with their specific sterol and lipid environment were shown to be important factors for protein recruitment into sub-compartmental structures of the plasma membrane. System-wide implications of altered endogenous sterol levels for membrane functions in living cells were not studied in higher plant cells. In particular, little is known how alterations in membrane sterol composition affect protein and lipid organization and interaction within membranes. Here, we conducted a comparative analysis of the plasma membrane protein and lipid composition in Arabidopsis sterol-biosynthesis mutants smt1 and ugt80A2;B1. smt1 shows general alterations in sterol composition while ugt80A2;B1 is significantly impaired in sterol glycosylation. By systematically analyzing different cellular fractions and combining proteomic with lipidomic data we were able to reveal contrasting alterations in lipid-protein interactions in both mutants, with resulting differential changes in plasma membrane signaling status. |
| Keywords: | Microdomains, Sterols, Sphingolipids, Signaling |
| Source: | Frontiers in Plant Science |
| ISSN: | 1664-462X |
| Publisher: | Frontiers Media SA |
| Volume: | 5 |
| Page Range: | 78 |
| Date: | 18 March 2014 |
| Official Publication: | https://doi.org/10.3389/fpls.2014.00078 |
| PubMed: | View item in PubMed |
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