Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Suicidal autointegration of Sleeping Beauty and piggyBac transposons in eukaryotic cells

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB

Item Type:Article
Title:Suicidal autointegration of Sleeping Beauty and piggyBac transposons in eukaryotic cells
Creators Name:Wang, Y. and Wang, J. and Devaraj, A. and Singh, M. and Jimenez Orgaz, A. and Chen, J.X. and Selbach, M. and Ivics, Z. and Izsvák, Z.
Abstract:Transposons are discrete segments of DNA that have the distinctive ability to move and replicate within genomes across the tree of life. 'Cut and paste' DNA transposition involves excision from a donor locus and reintegration into a new locus in the genome. We studied molecular events following the excision steps of two eukaryotic DNA transposons, Sleeping Beauty (SB) and piggyBac (PB) that are widely used for genome manipulation in vertebrate species. SB originates from fish and PB from insects; thus, by introducing these transposons to human cells we aimed to monitor the process of establishing a transposon-host relationship in a naïve cellular environment. Similarly to retroviruses, neither SB nor PB is capable of self-avoidance because a significant portion of the excised transposons integrated back into its own genome in a suicidal process called autointegration. Barrier-to-autointegration factor (BANF1), a cellular co-factor of certain retroviruses, inhibited transposon autointegration, and was detected in higher-order protein complexes containing the SB transposase. Increasing size sensitized transposition for autointegration, consistent with elevated vulnerability of larger transposons. Both SB and PB were affected similarly by the size of the transposon in three different assays: excision, autointegration and productive transposition. Prior to reintegration, SB is completely separated from the donor molecule and followed an unbiased autointegration pattern, not associated with local hopping. Self-disruptive autointegration occurred at similar frequency for both transposons, while aberrant, pseudo-transposition events were more frequently observed for PB.
Keywords:DNA Transposable Elements, DNA-Binding Proteins, Eukaryotic Cells, HeLa Cells, Human Genome, Nuclear Proteins, Suicide Transgenic Genes, Transposases
Source:PLoS Genetics
ISSN:1553-7390
Publisher:Public Library of Science (U.S.A.)
Volume:10
Number:3
Page Range:e1004103
Date:13 March 2014
Official Publication:https://doi.org/10.1371/journal.pgen.1004103
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library