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Regulation of aldosterone biosynthesis by adrenal renin is mediated through AT1 receptors in renin transgenic rats

Item Type:Article
Title:Regulation of aldosterone biosynthesis by adrenal renin is mediated through AT1 receptors in renin transgenic rats
Creators Name:Volpe, M. and Rubattu, S. and Gigante, G. and Ganten, D. and Porcellini, A. and Russo, R. and Romano, M. and Enea, I. and Lee, M.A. and Trimarco, B.
Abstract:The transgenic (TG) rat (mREN2)27 is characterized by overexpression of the additional mouse Ren-2d gene in the adrenal cortex with marked suppression of renal renin. We have previously shown that in salt-depleted TG rats enhanced activation of mineralocorticoid biosynthesis is associated with selective stimulation of adrenal renin. To investigate whether the local renin-angiotensin system regulates aldosterone biosynthesis in the adrenal cortex of TG rats, we studied the effects of the AT1-angiotensin subtype receptor antagonist DuP 753 on aldosterone production in 5-week-old TG rats during salt restriction. All the rats (n = 56) were shifted from regular chow to a diet containing only 0.04% NaCl for 1 week. The AT1-receptor antagonist DuP 753 (10 mg/kg per day in drinking water) was administered to 27 of these rats during low-salt diet. Subgroups of rats were killed at 0,4, and 7 days. Low-salt diet increased both adrenal renin activity (from 31 +/- 3 to 77 +/- 4 and 85 +/- 2 ng angiotensin I.h-1.mg protein-1 at 4 and 7 days, respectively; P < .001) and mRNA (by 68.4 +/- 10% and 80 +/- 17% from baseline, P < .05).
Keywords:Aldosterone, Angiotensin, Losartan, Renin, Transgenic Rats, Animals, Mice, Rats
Source:Circulation Research
ISSN:0009-7330
Publisher:American Heart Association (U.S.A.)
Volume:77
Number:1
Page Range:73-79
Date:July 1995
Official Publication:http://circres.ahajournals.org/cgi/content/abstract/77/1/73
PubMed:View item in PubMed

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