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An ES-like pluripotent state in FGF-dependent murine iPS cells

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Item Type:Article
Title:An ES-like pluripotent state in FGF-dependent murine iPS cells
Creators Name:Di Stefano, B. and Buecker, C. and Ungaro, F. and Prigione, A. and Chen, H.H. and Welling, M. and Eijpe, M. and Mostoslavsky, G. and Tesar, P. and Adjaye, J. and Geijsen, N. and Broccoli, V.
Abstract:Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions.
Keywords:Cell Culture Techniques, Embryo Culture Techniques, Embryonic Stem Cells, Fibroblast Growth Factors, Fibroblasts, Green Fluorescent Proteins, Induced Pluripotent Stem Cells, Intercellular Signaling Peptides and Proteins, Retroviridae, Species Specificity, Teratoma, Transgenic Mice, X Chromosome, Animals, Mice
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:5
Number:12
Page Range:e16092
Date:30 December 2010
Official Publication:https://doi.org/10.1371/journal.pone.0016092
PubMed:View item in PubMed

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