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Gatekeeper of pluripotency: a common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells

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Item Type:Article
Title:Gatekeeper of pluripotency: a common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells
Creators Name:Zuccotti, M. and Merico, V. and Bellone, M. and Mulas, F. and Sacchi, L. and Rebuzzini, P. and Prigione, A. and Redi, C.A. and Bellazzi, R. and Adjaye, J. and Garagna, S.
Abstract:BACKGROUND: Oct4 is a key factor of an expanded transcriptional network (Oct4-TN) that governs pluripotency and self-renewal in embryonic stem cells (ESCs) and in the inner cell mass from which ESCs are derived. A pending question is whether the establishment of the Oct4-TN initiates during oogenesis or after fertilisation. To this regard, recent evidence has shown that Oct4 controls a poorly known Oct4-TN central to the acquisition of the mouse egg developmental competence. The aim of this study was to investigate the identity and extension of this maternal Oct4-TN, as much as whether its presence is circumscribed to the egg or maintained beyond fertilisation. RESULTS: By comparing the genome-wide transcriptional profile of developmentally competent eggs that express the OCT4 protein to that of developmentally incompetent eggs in which OCT4 is down-regulated, we unveiled a maternal Oct4-TN of 182 genes. Eighty of these transcripts escape post-fertilisation degradation and represent the maternal Oct4-TN inheritance that is passed on to the 2-cell embryo. Most of these 80 genes are expressed in cancer cells and 37 are notable companions of the Oct4 transcriptome in ESCs. CONCLUSIONS: These results provide, for the first time, a developmental link between eggs, early preimplantation embryos and ESCs, indicating that the molecular signature that characterises the ESCs identity is rooted in oogenesis. Also, they contribute a useful resource to further study the mechanisms of Oct4 function and regulation during the maternal-to-embryo transition and to explore the link between the regulation of pluripotency and the acquisition of de-differentiation in cancer cells.
Keywords:Down-Regulation, Embryonic Stem Cells, Gene Expression Profiling, Gene Regulatory Networks, Multigene Family, Octamer Transcription Factor-3, Oligonucleotide Array Sequence Analysis, Oocytes, Animals, Mice
Source:BMC Genomics
ISSN:1471-2164
Publisher:BioMed Central (U.K.)
Volume:12
Page Range:345
Date:5 July 2011
Official Publication:https://doi.org/10.1186/1471-2164-12-345
PubMed:View item in PubMed

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