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| Item Type: | Article |
|---|---|
| Title: | Gatekeeper of pluripotency: a common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells |
| Creators Name: | Zuccotti, M. and Merico, V. and Bellone, M. and Mulas, F. and Sacchi, L. and Rebuzzini, P. and Prigione, A. and Redi, C.A. and Bellazzi, R. and Adjaye, J. and Garagna, S. |
| Abstract: | BACKGROUND: Oct4 is a key factor of an expanded transcriptional network (Oct4-TN) that governs pluripotency and self-renewal in embryonic stem cells (ESCs) and in the inner cell mass from which ESCs are derived. A pending question is whether the establishment of the Oct4-TN initiates during oogenesis or after fertilisation. To this regard, recent evidence has shown that Oct4 controls a poorly known Oct4-TN central to the acquisition of the mouse egg developmental competence. The aim of this study was to investigate the identity and extension of this maternal Oct4-TN, as much as whether its presence is circumscribed to the egg or maintained beyond fertilisation. RESULTS: By comparing the genome-wide transcriptional profile of developmentally competent eggs that express the OCT4 protein to that of developmentally incompetent eggs in which OCT4 is down-regulated, we unveiled a maternal Oct4-TN of 182 genes. Eighty of these transcripts escape post-fertilisation degradation and represent the maternal Oct4-TN inheritance that is passed on to the 2-cell embryo. Most of these 80 genes are expressed in cancer cells and 37 are notable companions of the Oct4 transcriptome in ESCs. CONCLUSIONS: These results provide, for the first time, a developmental link between eggs, early preimplantation embryos and ESCs, indicating that the molecular signature that characterises the ESCs identity is rooted in oogenesis. Also, they contribute a useful resource to further study the mechanisms of Oct4 function and regulation during the maternal-to-embryo transition and to explore the link between the regulation of pluripotency and the acquisition of de-differentiation in cancer cells. |
| Keywords: | Down-Regulation, Embryonic Stem Cells, Gene Expression Profiling, Gene Regulatory Networks, Multigene Family, Octamer Transcription Factor-3, Oligonucleotide Array Sequence Analysis, Oocytes, Animals, Mice |
| Source: | BMC Genomics |
| ISSN: | 1471-2164 |
| Publisher: | BioMed Central |
| Volume: | 12 |
| Page Range: | 345 |
| Date: | 5 July 2011 |
| Official Publication: | https://doi.org/10.1186/1471-2164-12-345 |
| PubMed: | View item in PubMed |
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