Caspase-dependent regulation and subcellular redistribution of the transcriptional modulator YY1 during apoptosis

Item Type:	Article
Title:	Caspase-dependent regulation and subcellular redistribution of the transcriptional modulator YY1 during apoptosis
Creators Name:	Krippner-Heidenreich, A. and Walsemann, G. and Beyrouthy, M.J. and Speckgens, S. and Kraft, R. and Thole, H. and Talanian, R.V. and Hurt, M.M. and Luescher, B.
Abstract:	The transcriptional regulator Yin Yang 1 (YY1) controls many aspects of cell behavior and is essential for development. We analyzed the fate of YY1 during apoptosis and studied the functional consequences. We observed that this factor is rapidly translocated into the cell nucleus in response to various apoptotic stimuli, including activation of Fas, stimulation by tumor necrosis factor, and staurosporine and etoposide treatment. Furthermore, YY1 is cleaved by caspases in vitro and in vivo at two distinct sites, IATD(12)G and DDSD(119)G, resulting in the deletion of the first 119 amino acids early in the apoptotic process. This activity generates an N-terminally truncated YY1 fragment (YY1{delta}119) that has lost its transactivation domain but retains its DNA binding domain. Indeed, YY1{delta}119 is no longer able to stimulate gene transcription but interacts with DNA. YY1{delta}119 but not the wild-type protein or the caspase-resistant mutant YY1D12A/D119A enhances Fas-induced apoptosis, suggesting that YY1 is involved in a positive feedback loop during apoptosis. Our findings provide evidence for a new mode of regulation of YY1 and define a novel aspect of the involvement of YY1 in the apoptotic process.
Keywords:	Apoptosis, Caspases, Cell Differentiation, Cell Line, Cell Nucleus, Cell Nucleus Active Transport, Cell Proliferation, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Genetic Transcription, Tertiary Protein Structure, Transcription Factors, YY1 Transcription Factor
Source:	Molecular and Cellular Biology
ISSN:	0270-7306
Publisher:	American Society for Microbiology
Volume:	25
Number:	9
Page Range:	3704-3714
Date:	May 2005
Official Publication:	https://doi.org/10.1128/MCB.25.9.3704-3714.2005
PubMed:	View item in PubMed

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