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| Item Type: | Article |
|---|---|
| Title: | The influence of Mn2+ on DNA structure in the presence of Na+ ions: a Raman spectroscopic study |
| Creators Name: | Muntean, C.M., Misselwitz, R. and Welfle, H. |
| Abstract: | The influence of Mn(2+) ions on the structure of natural calf thymus DNA was studied by Raman spectroscopy. Measurements were done at room temperature and pH 6.2±0.2, in the presence of the physiological concentration of 150 mM Na+ ions, and in the presence of Mn(2+) concentrations that varied between 0 and 600 mM. No condensation of DNA was observed at any of the Mn(2+) concentrations. At 5 mM Mn(2+) and 150 mM Na(+) no significant influence of Mn(2+) ions on the DNA structure can be observed. Compared with our previous results obtained at 10 mM Na(+) ions, binding of Mn(2+) ions to charged phosphate groups and to DNA bases is inhibited in the presence of 150 mM Na(+) ions. DNA backbone conformational changes were not observed in the whole concentration range of Mn(2+) ions as judging from the Raman spectra. No evidence for DNA melting was identified. A high Mn(2+) affinity for binding to guanine N7 and possibly, in a much lesser extent, to adenine have been found. |
| Keywords: | Manganese(II) Ions, Sodium Ions, DNA Structure, Raman Spectroscopy, Difference Spectra |
| Source: | Spectroscopy |
| ISSN: | 0712-4813 |
| Publisher: | Advanstar Communications |
| Volume: | 20 |
| Number: | 5-6 |
| Page Range: | 261-268 |
| Date: | 2006 |
| Official Publication: | https://doi.org/10.1155/2006/219603 |
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