Identification and characterization of a novel glucose-phosphorylating enzyme in Kluyveromyces lactis

Item Type:	Article
Title:	Identification and characterization of a novel glucose-phosphorylating enzyme in Kluyveromyces lactis
Creators Name:	Kettner, K. and Mueller, E.C. and Otto, A. and Roedel, G. and Breunig, K.D. and Kriegel, T.M.
Abstract:	Recent data suggest that hexokinase KlHxk1 (Rag5) represents the only glucose-phosphorylating enzyme of Kluyveromyces lactis, which also is required for glucose signalling. Long-term growth studies of a K. lactis rag5 mutant, however, reveal slow growth on glucose, but no growth on fructose. Isolation of the permissive glucose-phosphorylating enzyme, mass spectrometric tryptic peptide analysis and determination of basic kinetic data identify a novel glucokinase (KlGlk1) encoded by ORF KLLA0C01,155g. In accordance with the growth characteristics of the rag5 mutant, KlGlk1 phosphorylates glucose, but fails to act on fructose as a sugar substrate. Multiple sequence alignment indicates the presence of at least one glucokinase gene in all sequenced yeast genomes.
Keywords:	Glucokinase, Hexokinase, KlGlk1, KlHxk1, Kluyveromyces lactis, Substrate Specificity
Source:	FEMS Yeast Research
ISSN:	1567-1356
Publisher:	Blackwell Publishing
Volume:	7
Number:	5
Page Range:	683-692
Date:	August 2007
Official Publication:	https://doi.org/10.1111/j.1567-1364.2007.00259.x
PubMed:	View item in PubMed

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