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Live cell imaging of G protein-coupled receptors

Item Type:Article
Title:Live cell imaging of G protein-coupled receptors
Creators Name:Teichmann, A. and Schmidt, A. and Wiesner, B. and Oksche, A. and Schuelein, R.
Abstract:Live cell imaging experiments with G protein-coupled receptors (GPCRs) tagged with fluorescent fusion proteins were originally performed to study trafficking and subcellular location of these important drug targets. In the past decade, however, substantial progress came from improved imaging methods and from the cloning of novel fluorescent fusion proteins. Today, these methods allow to visualize not only GPCR interactions but also, e.g., receptor activation, trafficking between subcellular compartments, and to measure transport kinetics. Here, we summarize recent progress in live cell imaging of GPCRs using a confocal laser scanning microscope.
Keywords:G Protein-Coupled Receptors, Fluorescence Proteins, Subcellular Localization, Fluorescence Resonance Energy Transfer, Fluorescence Lifetime Imaging Microscopy, Fluorescence Correlation Spectroscopy
Source:Methods in Molecular Biology
ISSN:1064-3745
Publisher:Springer / Humana Press (U.S.A.)
Volume:897
Page Range:139-169
Date:2012
Official Publication:https://doi.org/10.1007/978-1-61779-909-9_7
PubMed:View item in PubMed

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