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Differential cAMP signaling at hippocampal output synapses

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Item Type:Article
Title:Differential cAMP signaling at hippocampal output synapses
Creators Name:Wozny, C. and Maier, N. and Fidzinski, P. and Breustedt, J. and Behr, J. and Schmitz, D.
Abstract:cAMP is a critical second messenger involved in synaptic transmission and synaptic plasticity. Here, we show that activation of the adenylyl cyclase by forskolin and application of the cAMP-analog Sp-5,6-DCl-cBIMPS both mimicked and occluded tetanus-induced long-term potentiation (LTP) in subicular bursting neurons, but not in subicular regular firing cells. Furthermore, LTP in bursting cells was inhibited by protein kinase A (PKA) inhibitors Rp-8-CPT-cAMP and H-89. Variations in the degree of EPSC blockade by the low-affinity competitive AMPA receptor-antagonist {gamma}-d-glutamyl-glycine ({gamma}-DGG), analysis of the coefficient of variance as well as changes in short-term potentiation suggest an increase of glutamate concentration in the synaptic cleft after expression of LTP. We conclude that presynaptic LTP in bursting cells requires activation of PKA by a calcium-dependent adenylyl cyclase while LTP in regular firing cells is independent of elevated cAMP levels. Our results provide evidence for a differential role of cAMP in LTP at hippocampal output synapses.
Keywords:Bursting, cAMP, Forskolin, LTP, Subiculum, Hippocampus, Animals, Rats
Source:Journal of Neuroscience
Publisher:Society for Neuroscience
Page Range:14358-14362
Date:31 December 2008
Additional Information:Copyright © 2008 Society for Neuroscience
Official Publication:https://doi.org/10.1523/JNEUROSCI.4973-08.2008
PubMed:View item in PubMed

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