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An approach for reliably investigating hippocampal sharp wave-ripples in vitro

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Item Type:Article
Title:An approach for reliably investigating hippocampal sharp wave-ripples in vitro
Creators Name:Maier, N. and Morris, G. and Johenning, F.W. and Schmitz, D.
Abstract:Background: Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the network function during complex activities like SPW-R, such knowledge is currently notably scarce. Methodology/Principal Findings: We demonstrate an in vitro approach to SPW-R that offers a simple experimental tool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperature is the required condition to preserve network integrity that is necessary for the generation of SPW-R. Moreover, we demonstrate the utility of our method for studying synaptic and network properties of SPW-R, using electrophysiological and imaging methods that can only be applied in the submerged system. Conclusions/Significance: The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generation of new insight into the network physiology underlying SPW-R.
Keywords:Biological Models, Calcium, Electrophysiology, Hippocampus, Nerve Net, Neurons, Perfusion, Polylysine, Synaptic Transmission, Temperature, Animals, Mice
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:4
Number:9
Page Range:e6925
Date:7 September 2009
Official Publication:https://doi.org/10.1371/journal.pone.0006925
PubMed:View item in PubMed

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