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Analysis of the puromycin finding site in the 70 S ribosome of Escherichia coli at the peptide level

Item Type:Article
Title:Analysis of the puromycin finding site in the 70 S ribosome of Escherichia coli at the peptide level
Creators Name:Bischof, O. and Kruft, V. and Wittmann-Liebold, B.
Abstract:Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level.
Keywords:Amino Acid Sequence, Binding Sites, Competitive Binding, Escherichia Coli, High Pressure Liquid Chromatography, Kinetics, Molecular Sequence Data, Peptide Fragments, Puromycin, Ribosomal Proteins, Ribosomes, Tritium
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:269
Number:28
Page Range:18315-18319
Date:15 July 1994
Official Publication:http://www.jbc.org/content/269/28/18315.abstract
PubMed:View item in PubMed

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