The role of CD8(+) T cells and their local interaction with CD4(+) T cells in myelin oligodendrocyte glycoprotein(35-55)-induced experimental autoimmune encephalomyelitis

Item Type:	Article
Title:	The role of CD8(+) T cells and their local interaction with CD4(+) T cells in myelin oligodendrocyte glycoprotein(35-55)-induced experimental autoimmune encephalomyelitis
Creators Name:	Leuenberger, T. and Paterka, M. and Reuter, E. and Herz, J. and Niesner, R.A. and Radbruch, H. and Bopp, T. and Zipp, F. and Siffrin, V.
Abstract:	T cells have an essential role in the induction of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Although for CD4(+) T cells it is well established that they contribute to the disease, less is known about the role of CD8(+) T cells. Our aim was to determine the individual contribution of CD4(+) and CD8(+) T cells in myelin oligodendrocyte glycoprotein (MOG)35-55-induced EAE. We investigated MOG35-55-activated CD8(+) T cells to clarify their potential to induce or attenuate EAE. We monitored the behavior of CD8(+) T cells and their interaction with CD4(+) T cells directly at the site of inflammation in the CNS using intravital imaging of the brainstem of EAE-affected living anesthetized mice. We found that mice without CD4(+) T cells did not develop relevant clinical signs of disease, although CD8(+) T cells were present in the CNS of these mice. These CD8(+) T cells displayed reduced motility compared with those in the presence of CD4(+) T cells. In mice that harbored CD4(+) and CD8(+) T cells, we saw a similar extent of clinical signs of EAE as in mice with only CD4(+) T cells. Furthermore, the dynamic motility and viability of CD4(+) T cells were not disturbed by CD8(+) T cells in the lesions of these mice. Therefore, we conclude that in MOG35-55-induced EAE, CD8(+) T cell accumulation in the CNS represents instead an epiphenomenon with no impact on clinical disease or on the effects of CD4(+) T cells, the latter being the true inducers of the disease.
Keywords:	CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Communication, Cell Movement, Central Nervous System, Experimental Autoimmune Encephalomyelitis, Inflammation, Lymphocyte Activation, Myelin-Oligodendrocyte Glycoprotein, Peptide Fragments, Animals, Mice
Source:	Journal of Immunology
ISSN:	0022-1767
Publisher:	American Association of Immunologists
Volume:	191
Number:	10
Page Range:	4960-4968
Date:	15 November 2013
Official Publication:	https://doi.org/10.4049/jimmunol.1300822
PubMed:	View item in PubMed

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