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Protein composition of the human heart: the construction of a myocardial two-dimensional electrophoresis database

Item Type:Article
Title:Protein composition of the human heart: the construction of a myocardial two-dimensional electrophoresis database
Creators Name:Jungblut, P. and Otto, A. and Zeindl-Eberhart, E. and Pleissner, K.P. and Knecht, M. and Regitz-Zagrosek, V. and Fleck, E. and Wittmann-Liebold, B.
Abstract:Molecular changes occurring in myocardial diseases are not well understood. Proteins, as regulatory molecules, should play an important role in the etiology of these diseases. The method of two-dimensional electrophoresis (2-DE) allows the analysis of some thousand proteins with one experiment. An important prerequisite for this kind of investigation is the possibility of identifying the proteins separated by 2-DE. We resolve 3239 proteins of the human myocardium and tried to identify 33 proteins by amino acid analysis and microsequencing. Twenty proteins were identified by search for the protein-chemical data obtained in the Martinsried Institute Protein Sequence Database. Comparisons of 2-DE patterns of different size, which were obtained in different laboratories, were performed with the result that proteins identified on a 2-DE map of one laboratory can be assigned to spots of 2-DE maps produced by another laboratory. Our results show the usefulness of a myocardial 2-DE database; they can be used in different laboratories and make it possible to generate a collection of important human myocardial proteins in a 2-DE database for comparative studies worldwide.
Keywords:Amino Acid Sequence, Amino Acids, Autoanalysis, Dilated Cardiomyopathy, Factual Databases, Gel, Two-Dimensional Electrophoresis, Isoelectric Focusing, Laboratories, Molecular Sequence Data, Myocardium, Proteins
Source:Electrophoresis
ISSN:0173-0835
Publisher:Wiley (Germany)
Volume:15
Number:5
Page Range:685-707
Date:1 May 1994
Official Publication:https://doi.org/10.1002/elps.1150150197
PubMed:View item in PubMed

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