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Acetylsalicylic acid reduces the severity of dextran sodium sulfate-induced colitis and increases the formation of anti-inflammatory lipid mediators

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Item Type:Article
Title:Acetylsalicylic acid reduces the severity of dextran sodium sulfate-induced colitis and increases the formation of anti-inflammatory lipid mediators
Creators Name:Koehnke, T. and Gomolka, B. and Bilal, S. and Zhou, X. and Sun, Y. and Rothe, M. and Baumgart, D.C. and Weylandt, K.H.
Abstract:The role of non-steroidal anti-inflammatory drugs in inflammatory bowel disease is controversial, as they have been implicated in disease aggravation. Different from other cyclooxygenase inhibitors, acetylsalicylic acid (ASA) enhances the formation of anti-inflammatory and proresolution lipoxins derived from arachidonic acid as well as resolvins from omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA). In this study, we examined the effect of ASA on murine dextran sodium sulfate colitis. A mouse magnetic resonance imaging (MRI) protocol and post mortem assessment were used to assess disease severity, and lipid metabolites were measured using liquid chromatography-coupled tandem mass spectrometry. Decreased colitis activity was demonstrated by phenotype and MRI assessment in mice treated with ASA, and confirmed in postmortem analysis. Analysis of lipid mediators showed sustained formation of lipoxin A4 and an increase of DHA-derived 17-hydroxydocosahexaenoic acid (17-HDHA) after treatment with ASA. Furthermore, in vitro experiments in RAW264.7 murine macrophages demonstrated significantly increased phagocytosis activity after incubation with 17-HDHA, supporting its proresolution effect. These results show a protective effect of ASA in a murine colitis model and could give a rationale for a careful reassessment of ASA therapy in patients with inflammatory bowel disease and particularly ulcerative colitis, possibly combined with DHA supplementation.
Keywords:Anti-Inflammatory Agents, Aspirin, Colitis, Dextran Sulfate, Docosahexaenoic Acids, Lipids, Magnetic Resonance Imaging, Inbred C57BL Mice, Phagocytosis, Animals, Mice
Source:BioMed Research International
ISSN:2314-6133
Publisher:Hindawi (U.S.A.)
Volume:2013
Page Range:748160
Date:8 September 2013
Official Publication:https://doi.org/10.1155/2013/748160
PubMed:View item in PubMed

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