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Ontogeny of excitatory spinal neurons processing distinct somatic sensory modalities

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Item Type:Article
Title:Ontogeny of excitatory spinal neurons processing distinct somatic sensory modalities
Creators Name:Xu, Y. and Lopes, C. and Wende, H. and Guo, Z. and Cheng, L. and Birchmeier, C. and Ma, Q.
Abstract:Spatial and temporal cues govern the genesis of a diverse array of neurons located in the dorsal spinal cord, including dI1-dI6, dILA, and dILB subtypes, but their physiological functions are poorly understood. Here we generated a new line of conditional knock-out (CKO) mice, in which the homeobox gene Tlx3 was removed in dI5 and dILB cells. In these CKO mice, development of a subset of excitatory neurons located in laminae I and II was impaired, including itch-related GRPR-expressing neurons, PKC{gamma}-expressing neurons, and neurons expressing three neuropeptide genes: somatostatin, preprotachykinin 1, and the gastrin-releasing peptide. These CKO mice displayed marked deficits in generating nocifensive motor behaviors evoked by a range of pain-related or itch-related stimuli. The mutants also failed to exhibit escape response evoked by dynamic mechanical stimuli but retained the ability to sense innocuous cooling and/or warm. Thus, our studies provide new insight into the ontogeny of spinal neurons processing distinct sensory modalities.
Keywords:Capsaicin, Cell Count, Chloroquine, Developmental Gene Expression Regulation, Gastrin-Releasing Peptide, Homeodomain Proteins, Mammalian Embryo, Muscle Proteins, Neurons, Oligopeptides, Pain, Physical Stimulation, Protein Kinase C, Protein Precursors, Pruritus, Psychomotor Performance, Sensation, Somatostatin, Spinal Ganglia, Tachykinins, Vesicular Glutamate Transport Protein 1, Animals, Mice
Source:Journal of Neuroscience
ISSN:0270-6474
Publisher:Society for Neuroscience (U.S.A.)
Volume:33
Number:37
Page Range:14738-14748
Date:11 September 2013
Official Publication:https://doi.org/10.1523/JNEUROSCI.5512-12.2013
PubMed:View item in PubMed

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