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Advances in imaging the interphase nucleus using thin cryosections

Item Type:Review
Title:Advances in imaging the interphase nucleus using thin cryosections
Creators Name:Pombo, A.
Abstract:The mammalian genome is partitioned amongst various chromosomes and encodes for approximately 30,000 protein-coding genes. Gene expression occurs after exit from mitosis, when chromosomes partially decondense within the cell nucleus to allow the enzymatic activities that work on chromatin to access each gene in a regulated fashion. Differential patterns of gene expression evolve during cell differentiation to give rise to the over 200 cell types in higher eukaryotes. The architectural organisation of the genome inside the interphase cell nucleus, and associated enzymatic activities, reveals dynamic and functional compartmentalization of the genome. In this review, I highlight the advantages of Tokuyasu cryosectioning on the investigation of nuclear structure and function.
Keywords:Nuclear Compartments, Gene Expression, Chromosome Territories, Transcription Factories, Confocal Microscopy, Correlative Microscopy, Animals
Source:Histochemistry and Cell Biology
Page Range:97-104
Date:August 2007
Official Publication:https://doi.org/10.1007/s00418-007-0310-x
PubMed:View item in PubMed

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