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Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment

Item Type:Article
Title:Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment
Creators Name:Alder, O. and Lavial, F. and Helness, A. and Brookes, E. and Pinho, S. and Chandrashekran, A. and Arnaud, P. and Pombo, A. and O'Neill, L. and Azuara, V.
Abstract:Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic of cells surrounded by an extra-embryonic layer, the trophectoderm. We show that a set of somatic lineage regulators (including Hox, Gata and Sox factors) that carry bivalent chromatin enriched in H3K27me3 and H3K4me2 are selectively targeted by Suv39h1-mediated H3K9me3 and de novo DNA methylation in extra-embryonic versus embryonic (pluripotent) lineages, as assessed both in blastocyst-derived stem cells and in vivo. This stably repressed state is linked with a loss of gene priming for transcription through the exclusion of PRC1 (Ring1B) and RNA polymerase II complexes at bivalent, lineage-inappropriate genes upon trophoblast lineage commitment. Collectively, our results suggest a mutually exclusive role for Ring1B and Suv39h1 in regulating distinct chromatin states at key developmental genes and propose a novel mechanism by which lineage specification can be reinforced during early development.
Keywords:Bivalent Chromatin, Early Development, Histone Methylation, Mouse, Silencing, Stem Cells, Animals, Mice
Source:Development
ISSN:0950-1991
Publisher:Company of Biologists
Volume:137
Number:15
Page Range:2483-2492
Date:1 August 2010
Official Publication:https://doi.org/10.1242/dev.048363
PubMed:View item in PubMed

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