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Measurement of ERK 1/2 in CSF from patients with neuropsychiatric disorders and evidence for the presence of the activated form

Item Type:Article
Title:Measurement of ERK 1/2 in CSF from patients with neuropsychiatric disorders and evidence for the presence of the activated form
Creators Name:Klafki, H.W. and Lewczuk, P. and Kamrowski-Kruck, H. and Maler, J.M. and Mueller, K. and Peters, O. and Heuser, I. and Jessen, F. and Popp, J. and Froelich, L. and Wolf, S. and Prinz, B. and Luckhaus, C. and Schroeder, J. and Pantel, J. and Gertz, H.J. and Koelsch, H. and Mueller, B.W. and Esselmann, H. and Bibl, M. and Kornhuber, J. and Wiltfang, J.
Abstract:The clinical diagnosis of neurodegenerative disorders can be supported by soluble biomarkers in cerebrospinal fluid (CSF), such as tau protein, phospho-tau, and amyloid-beta peptides. In particular, increased CSF levels of phospho-tau in Alzheimer's disease appear to reflect disease specific pathological processes. We report here evidence for the presence of soluble MAP-kinase ERK1/2 in a small set of human CSF samples from patients with Alzheimer's disease, frontotemporal degeneration, and mild cognitive impairment. The level of total ERK1/2 in CSF as measured by electrochemiluminescent assay was correlated with that of total tau and phospho-tau. A small fraction of ERK1/2 in a pooled CSF sample was found to be in the doubly phosphorylated (activated) state. Our findings suggest that i) MAP kinase ERK1/2 is apparently released under neurodegenerative conditions in parallel with tau and phospho-tau and ii) in the future, it might be possible to find in CSF samples evidence for disease related alterations in brain kinase signaling pathways by use of highly sensitive and activation-state specific anti-kinase antibodies.
Keywords:Alzheimer's Disease, Cerebrospinal Fluid, ERK1/2, Frontotemporal Degeneration, MAP Kinase, Mild Cognitive Impairment, Tau
Source:Journal of Alzheimer's Disease
ISSN:1387-2877
Publisher:IOS Press (The Netherlands)
Volume:18
Number:3
Page Range:613-622
Date:2009
Official Publication:https://doi.org/10.3233/JAD-2009-1167
PubMed:View item in PubMed

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