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Utilization of TREC and KREC quantification for the monitoring of early T- and B-cell neogenesis in adult patients after allogeneic hematopoietic stem cell transplantation

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Item Type:Article
Title:Utilization of TREC and KREC quantification for the monitoring of early T- and B-cell neogenesis in adult patients after allogeneic hematopoietic stem cell transplantation
Creators Name:Mensen, A. and Ochs, C. and Stroux, A. and Wittenbecher, F. and Szyska, M. and Imberti, L. and Fillatreau, S. and Uharek, L. and Arnold, R. and Dörken, B. and Thiel, A. and Scheibenbogen, C. and Na, I.K.
Abstract:BACKGROUND: After hematopoietic stem cell transplantation (HSCT) T- and B-cell reconstitution from primary lymphoid organs are a prerequisite for an effective early lymphocyte reconstitution and a long-term survival for adult patients suffering from acute leukemia. Here, we asked whether quantification of T cell receptor excision circle, (TREC) and kappa-deleting recombination excision circle (KREC) before and within six month after allogeneic HSCT could be used to measure the thymic and bone marrow outputs in such patients. METHODS: We used a duplex real time PCR assay to quantify the absolute copy counts of TREC and KREC, and correlated the data with absolute cell counts of CD3+CD4+ T-cell and CD19+ B-cell subsets determined by flow cytometry, respectively. RESULTS: By comparing two recently proposed naive T cell subsets, CD31+ naive and CD31- naive T cells, we found a better correlation for the CD31+ subset with TREC level post alloHSCT, in line with the assumption that it contained T cells recently derived from the thymus, indicating that TREC levels reflected real thymic de novo production. Transitional as well as naive B cells highly correlated with KREC levels, which suggested an association of KREC levels with ongoing bone marrow B cell output. CD45RO+ memory T cells and CD27+ memory B cells were significantly less correlated with TREC and KREC recovery, respectively. CONCLUSION: We conclude that simultaneous TREC/ KREC quantification is as a suitable and practicable method to monitor thymic and bone marrow output post alloHSCT in adult patients diagnosed with acute leukemia.
Keywords:Allogeneic Hematopoietic Stem Cell Transplantation, Acute Leukemia, Simultaneous TREC/KREC Quantification Assay, Monitoring Immune Reconstitution
Source:Journal of Translational Medicine
ISSN:1479-5876
Publisher:BioMed Central (U.K.)
Volume:11
Number:1
Page Range:188
Date:14 August 2013
Official Publication:https://doi.org/10.1186/1479-5876-11-188
PubMed:View item in PubMed

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