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Bi-PROF: bisulfite profiling of target regions using 454 GS FLX Titanium technology

Item Type:Article
Title:Bi-PROF: bisulfite profiling of target regions using 454 GS FLX Titanium technology
Creators Name:Gries, J. and Schumacher, D. and Arand, J. and Lutsik, P. and Markelova, M.R. and Fichtner, I. and Walter, J. and Sers, C. and Tierling, S.
Abstract:The use of next generation sequencing has expanded our view on whole mammalian methylome patterns. In particular, it provides a genome-wide insight of local DNA methylation diversity at single nucleotide level and enables the examination of single chromosome sequence sections at a sufficient statistical power. We describe a bisulfite-based sequence profiling pipeline, Bi-PROF, which is based on the 454 GS-FLX Titanium technology that allows to obtain up to one million sequence stretches at single base pair resolution without laborious subcloning. To illustrate the performance of the experimental workflow connected to a bioinformatics program pipeline (BiQ Analyzer HT) we present a test analysis set of 68 different epigenetic marker regions (amplicons) in five individual patient-derived xenograft tissue samples of colorectal cancer and one healthy colon epithelium sample as a control. After the 454 GS-FLX Titanium run, sequence read processing and sample decoding, the obtained alignments are quality controlled and statistically evaluated. Comprehensive methylation pattern interpretation (profiling) assessed by analyzing 10 ( 2) -10 ( 4) sequence reads per amplicon allows an unprecedented deep view on pattern formation and methylation marker heterogeneity in tissues concerned by complex diseases like cancer.
Keywords:BiQ Analyzer HT, DNA Methylation, Bisulfite Profiling, Colorectal Cancer, Next-Generation Sequencing, Animals, Mice
Publisher:Landes Bioscience
Page Range:765-771
Date:July 2013
Official Publication:https://doi.org/10.4161/epi.25242
PubMed:View item in PubMed

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