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Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation

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Item Type:Article
Title:Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation
Creators Name:Liu, Y. and Hu, W. and Murakawa, Y. and Yin, J. and Wang, G. and Landthaler, M. and Yan, J.
Abstract:The body temperature is considered a universal cue by which the master clock synchronizes the peripheral clocks in mammals, but the mechanism is not fully understood. Here we identified two cold-induced RNA-binding proteins (RBPs), Cirbp and Rbm3, as important regulators for the temperature entrained circadian gene expression. The depletion of Cirbp or Rbm3 significantly reduced the amplitudes of core circadian genes. PAR-CLIP analyses showed that the 3'UTR binding sites of Cirbp and Rbm3 were significantly enriched near the polyadenylation sites (PASs). Furthermore, the depletion of Cirbp or Rbm3 shortened 3'UTR, whereas low temperature (upregulating Cirbp and Rbm3) lengthened 3'UTR. Remarkably, we found that they repressed the usage of proximal PASs by binding to the common 3'UTR, and many cases of proximal/distal PAS selection regulated by them showed strong circadian oscillations. Our results suggested that Cirbp and Rbm3 regulated the circadian gene expression by controlling alternative polyadenylation (APA).
Keywords:Gene Regulatory Networks, Alternative Splicing, RNA, Circadian Rhythms, Animals, Mice
Source:Scientific Reports
ISSN:2045-2322
Publisher:Nature Publishing Group
Volume:3
Page Range:2054
Date:2013
Official Publication:https://doi.org/10.1038/srep02054
PubMed:View item in PubMed

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