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ECM modulated early kidney development in embryonic organ culture

Item Type:Article
Title:ECM modulated early kidney development in embryonic organ culture
Creators Name:Sebinger, D.D. and Ofenbauer, A. and Gruber, P. and Malik, S. and Werner, C.
Abstract:The use of exogenous signals is gaining importance in renal regenerative therapies. We wanted to explore the role of extracellular matrix (ECM) constituents on renal structure formation during renal organogenesis. We used a recently established organ culture setup to expose embryonic kidney rudiments directly to a large set of surface-immobilized or dissolved ECM molecules and growth factors. Organ culture was also performed on immobilized adult kidney ECM extracts and on reactive polymer films without any biomolecular components. The applied conditions resulted in distinct differences of organ phenotypes, underlining the multifaceted role of exogenous signals during kidney development. Specific ECM components, including collagen I and laminin, supported nephronal and tubular structure formation of the developing organ. ECM biopolymers, e.g. hyaluronic acid, were found to determine the fate of developing explants in a concentration- and molecular weight-dependent manner. The organ culture system used was an effective and robust means to identify exogenous signals that direct kidney development. This system can provide valuable insight for future regenerative therapies of kidney diseases.
Keywords:Organ Culture, Embryonic Kidney Rudiments, ECM (Extracellular Matrix), Kidney Tissue Engineering, Hyaluronic Acid, Nephron, Animals, Mice
Page Range:6670-6682
Date:September 2013
Official Publication:https://doi.org/10.1016/j.biomaterials.2013.05.031
PubMed:View item in PubMed

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