Helmholtz Gemeinschaft


A new light source for multimodal multiphoton microscopy including CARS

Item Type:Conference or Workshop Item
Title:A new light source for multimodal multiphoton microscopy including CARS
Creators Name:Rimke, I. and Siffrin, V. and Niesner, R. and Leuenberger, T. and Zipp, F. and Buttnera, E. and Stibenza, G.
Abstract:We will present a new flexible laser source for multimodal Multiphoton excitation microscopy including CARS. It consists of a tuneable femtosecond-Ti:Sapphire laser and an optical parametric oscillator (OPO). The new OPO-design allows for high flexibility in pump- and output wavelengths giving rise to for instance image EGFP with the Ti:Sapphire and tdRFP with the OPO simultaneously. This is presented on living mouse brain tissue. The minimum energy difference between Ti:Sapphire and OPO-wavelengths achievable is 2500cm-1. Thus CARS imaging of lipids is possible. Due to synchronous pumping of the OPO the pump- and OPO pulses are intrinsically locked in time to each other thus they can be brought to perfect overlap of pump and stokes pulses. Uncaging multiphoton microscopy is also possible with this system due to the low minimum OPO pump wavelength of 730nm.
Keywords:Brain, CARS Tomography, Gain Switching, Laser Sources, Light Sources, Microscopy, Multiphoton Microscopy, Optical Parametric Oscillators, Sapphire, Sapphire Lasers
Source:Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher:International Society for Optical Engineering
Page Range:718314
Date:13 February 2009
Official Publication:https://doi.org/10.1117/12.808376

Repository Staff Only: item control page

Open Access
MDC Library