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Generation of rat induced pluripotent stem cells: the analysis of reprogramming and culturing media

Item Type:Article
Title:Generation of rat induced pluripotent stem cells: the analysis of reprogramming and culturing media
Creators Name:Liskovykh, M.A. and Chuykin, I.A. and Ranjan, A. and Safina, D.A. and Tolkunova, E.N. and Minina, Y.M. and Zhdanova, N.S. and Dyban, P.A, and Mullins, J. and Kostyleva, E.I. and Chikhirzhina, E.V. and Bader, M. and Alenina, N. and Tomilin, A.N.
Abstract:The rat represents very important, superior in many respects to the mous, animal model for studying pharmacology, physiology, ageing, cardiovascular etc. However, numerous attempts to derive rat ES cells necessary to carry out loss-of-gene-function studies have not been successful thus far. Therefore rat induct pluripotent stem cells (or riPS) should provide a notable alternative to ES cell, allowing to study gene functions in this valuable animal model. Here we report an improved lentivirus-based riPS derivation protocol that makes use of small inhibitors of MEK and GSK3. We show that the excision of proviruses does not affect neither karyotype and pluripotency state of these cells. Also, we propose genetic tool for an improvement of the quality of riPS cells in culture. These data may prompt further iPS-based gene targeting in rat as well as the development iPS-based gene therapies, using this animal model.
Keywords:Induced Pluripotency Stem Cells, Reprogramming, Animals, Rats
Publisher:MAIK Nauka/Interperiodica
Page Range:939-945
Official Publication:https://doi.org/10.1134/S1990519X12020095
PubMed:View item in PubMed

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