KSHV ORF67 encoded lytic protein localizes on the nuclear membrane and alters emerin distribution

Item Type:	Article
Title:	KSHV ORF67 encoded lytic protein localizes on the nuclear membrane and alters emerin distribution
Creators Name:	Farina, A. and Santarelli, R. and Bloise, R. and Gonnella, R. and Granato, M. and Bei, R. and Modesti, A. and Cirone, M. and Bengtsson, L. and Angeloni, A. and Faggioni, A.
Abstract:	p29, a newly identified Kaposi's sarcoma-associated herpesvirus (KSHV) protein, is the product of ORF67, the positional homolog of the conserved herpesvirus protein UL34. Like its homologues in other herpesviruses, p29 is expressed early during viral lytic cycle, and is localized on the nuclear rim. Upon chemical induction of viral replication in primary effusion lymphoma cells, p29 interacts with p33, encoded by ORF69, the positional homolog of the conserved herpesvirus protein UL31, and both proteins colocalize on the nuclear membrane. IFA and biochemical analysis of infected or transfected cells showed that p29 expression resulted in delocalization and hyperphosphorylation of emerin, whereas other nuclear lamin associated proteins, such as LUMA, LB1 and LBR were not affected. Mislocalization of emerin was robustly increased upon combined expression of p29 and p33, suggesting that emerin destabilization might represent the first step in nuclear lamina disassembling, a process necessary for nucleocapsid maturation.
Keywords:	KSHV, ORF67, ORF69, Viral Egress, Nuclear Envelope
Source:	Virus Research
ISSN:	0168-1702
Publisher:	Elsevier
Volume:	175
Number:	2
Page Range:	143-150
Date:	August 2013
Official Publication:	https://doi.org/10.1016/j.virusres.2013.04.001
PubMed:	View item in PubMed

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