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Large-scale mapping of mutations affecting zebrafish development

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Item Type:Article
Title:Large-scale mapping of mutations affecting zebrafish development
Creators Name:Geisler, R. and Rauch, G.J. and Geiger-Rudolph, S. and Albrecht, A. and van Bebber, F. and Berger, A. and Busch-Nentwich, E. and Dahm, R. and Dekens, M.P. and Dooley, C. and Elli, A.F. and Gehring, I. and Geiger, H. and Geisler, M. and Glaser, S. and Holley, S. and Huber, M. and Kerr, A. and Kirn, A. and Knirsch, M. and Konantz, M. and Kuechler, A.M. and Maderspacher, F. and Neuhauss, S.C. and Nicolson, T. and Ober, E.A. and Praeg, E. and Ray, R. and Rentzsch, B. and Rick, J.M. and Rief, E. and Schauerte, H.E. and Schepp, C.P. and Schoenberger, U. and Schonthaler, H.B. and Seiler, C. and Sidi, S. and Soellner, C. and Wehner, A. and Weiler, C. and Nuesslein-Volhard, C.
Abstract:Background: Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers. Results: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tuebingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM. Conclusion: By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.
Keywords:Chromosome Mapping, Genome, Microsatellite Repeats, Mutagenesis, Mutation, Phenotype, Animals, Zebrafish
Source:BMC Genomics
Publisher:BioMed Central
Page Range:11
Date:9 January 2007
Official Publication:https://doi.org/10.1186/1471-2164-8-11
PubMed:View item in PubMed

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