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Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots

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Item Type:Article
Title:Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots
Creators Name:Sporbert, A. and Cseresnyes, Z. and Heidbreder, M. and Domaing, P. and Hauser, S. and Kaltschmidt, B. and Kaltschmidt, C. and Heilemann, M. and Widera, D.
Abstract:This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
Keywords:Cell Line, Confocal Microscopy, Lasers, Photons, Quantum Dots, Three-Dimensional Imaging, Animals, Mice
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:8
Number:5
Page Range:e64023
Date:21 May 2013
Official Publication:https://doi.org/10.1371/journal.pone.0064023
PubMed:View item in PubMed

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