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| Item Type: | Article |
|---|---|
| Title: | Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots |
| Creators Name: | Sporbert, A. and Cseresnyes, Z. and Heidbreder, M. and Domaing, P. and Hauser, S. and Kaltschmidt, B. and Kaltschmidt, C. and Heilemann, M. and Widera, D. |
| Abstract: | This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts. |
| Keywords: | Cell Line, Confocal Microscopy, Lasers, Photons, Quantum Dots, Three-Dimensional Imaging, Animals, Mice |
| Source: | PLoS ONE |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Volume: | 8 |
| Number: | 5 |
| Page Range: | e64023 |
| Date: | 21 May 2013 |
| Official Publication: | https://doi.org/10.1371/journal.pone.0064023 |
| PubMed: | View item in PubMed |
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