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A functional deficiency of TERA/VCP/p97 contributes to impaired DNA repair in multiple polyglutamine diseases

Official URL:https://doi.org/10.1038/ncomms2828
PubMed:View item in PubMed
Creators Name:Fujita, K. and Nakamura, Y. and Oka, T. and Ito, H. and Tamura, T. and Tagawa, K. and Sasabe, T. and Katsuta, A. and Motoki, K. and Shiwaku, H. and Sone, M. and Yoshida, C. and Katsuno, M. and Eishi, Y. and Murata, M. and Taylor, J.P. and Wanker, E.E. and Kono, K. and Tashiro, S. and Sobue, G. and La Spada, A.R. and Okazawa, H.
Journal Title:Nature Communications
Journal Abbreviation:Nat Commun
Volume:4
Page Range:1816
Date:2013
Keywords:Adenosine Triphosphatases, Cell Cycle Proteins, Cerebral Cortex, DNA Repair, Double-Stranded DNA Breaks, Endoplasmic Reticulum, Green Fluorescent Proteins, HEK293 Cells, HeLa Cells, Histones, Immunoprecipitation, Inclusion Bodies, Longevity, Motor Neurons, Mutant Proteins, Nerve Tissue Proteins, Nuclear Proteins, Peptides, Phenotype, Protein Binding, Protein Transport, Animals, Drosophila melanogaster, Mice
Abstract:It is hypothesized that a common underlying mechanism links multiple neurodegenerative disorders. Here we show that transitional endoplasmic reticulum ATPase (TERA)/valosin-containing protein (VCP)/p97 directly binds to multiple polyglutamine disease proteins (huntingtin, ataxin-1, ataxin-7 and androgen receptor) via polyglutamine sequence. Although normal and mutant polyglutamine proteins interact with TERA/VCP/p97, only mutant proteins affect dynamism of TERA/VCP/p97. Among multiple functions of TERA/VCP/p97, we reveal that functional defect of TERA/VCP/p97 in DNA double-stranded break repair is critical for the pathology of neurons in which TERA/VCP/p97 is located dominantly in the nucleus in vivo. Mutant polyglutamine proteins impair accumulation of TERA/VCP/p97 and interaction of related double-stranded break repair proteins, finally causing the increase of unrepaired double-stranded break. Consistently, the recovery of lifespan in polyglutamine disease fly models by TERA/VCP/p97 corresponds well to the improvement of double-stranded break in neurons. Taken together, our results provide a novel common pathomechanism in multiple polyglutamine diseases that is mediated by DNA repair function of TERA/VCP/p97.
ISSN:2041-1723
Publisher:Nature Publishing Group (U.K.)
Item Type:Article

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