Helmholtz Gemeinschaft


Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program
Creators Name:Knapp, D. and Schulz, H. and Rascon, C.A. and Volkmer, M. and Scholz, J. and Nacu, E. and Le, M. and Novozhilov, S. and Tazaki, A. and Protze, S. and Jacob, T. and Hubner, N. and Habermann, B. and Tanaka, E.M.
Abstract:Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression - early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation.
Keywords:Amphibian Proteins, Cluster Analysis, Extremities, Gene Expression Profiling, Gene Expression Regulation, Gene Ontology, Oligonucleotide Array Sequence Analysis, Regeneration, Physiological Stress, Transcriptome, Up-Regulation, Wound Healing, Animals, Ambystoma mexicanum
Source:PLoS ONE
Publisher:Public Library of Science
Page Range:e61352
Date:1 May 2013
Official Publication:https://doi.org/10.1371/journal.pone.0061352
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library