Helmholtz Gemeinschaft


Widespread splicing changes in human brain development and aging

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:Widespread splicing changes in human brain development and aging
Creators Name:Mazin, P. and Xiong, J. and Liu, X. and Yan, Z. and Zhang, X. and Li, M. and He, L. and Somel, M. and Yuan, Y. and Chen, Y.P.P. and Li, N. and Hu, Y. and Fu, N. and Ning, Z. and Zeng, R. and Yang, H. and Chen, W. and Gelfand, M. and Khaitovich, P.
Abstract:While splicing differences between tissues, sexes and species are well documented, little is known about the extent and the nature of splicing changes that take place during human or mammalian development and aging. Here, using high-throughput transcriptome sequencing, we have characterized splicing changes that take place during whole human lifespan in two brain regions: prefrontal cortex and cerebellum. Identified changes were confirmed using independent human and rhesus macaque RNA-seq data sets, exon arrays and PCR, and were detected at the protein level using mass spectrometry. Splicing changes across lifespan were abundant in both of the brain regions studied, affecting more than a third of the genes expressed in the human brain. Approximately 15% of these changes differed between the two brain regions. Across lifespan, splicing changes followed discrete patterns that could be linked to neural functions, and associated with the expression profiles of the corresponding splicing factors. More than 60% of all splicing changes represented a single splicing pattern reflecting preferential inclusion of gene segments potentially targeting transcripts for nonsense-mediated decay in infants and elderly.
Keywords:Alternative Splicing, Brain, Development, Human, RNA-Seq, Animals
Source:Molecular Systems Biology
Publisher:Nature Publishing Group
Page Range:633
Official Publication:https://doi.org/10.1038/msb.2012.67
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library