Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Dorsal-ventral pattern of Delta trafficking is established by a Snail-Tom-Neuralized pathway

Item Type:Article
Title:Dorsal-ventral pattern of Delta trafficking is established by a Snail-Tom-Neuralized pathway
Creators Name:De Renzis, S. and Yu, J. and Zinzen, R.P. and Wieschaus, E.
Abstract:The intracellular trafficking of the Notch ligand Delta plays an important role in the activation of the Notch pathway. We have addressed the snail-dependent regulation of Delta trafficking during the plasma membrane growth of the mesoderm in the Drosophila embryo. We show that Delta is retained in endocytic vesicles in the mesoderm but expressed on the surface of the adjacent ectoderm. This trafficking pattern requires Neuralized. We developed a protocol based on chromosomal deletion and microarray analysis that led to the identification of tom as the target of snail regulating Delta trafficking. Snail represses Tom expression in the mesoderm and thereby activates Delta trafficking. Overexpression of Tom abolishes Delta trafficking and signaling to the adjacent mesoectoderm. Loss of Tom produces mesoderm-type Delta trafficking in the entire blastoderm epithelium and an expansion of mesoectoderm gene expression. We propose that Tom antagonizes the activity of Neuralized and thus establishes a sharp mesoderm-mesoectoderm boundary of Notch signaling.
Keywords:SIGNALING, DEVBIO, Body Patterning, Chromosome Mapping, DNA-Binding Proteins, Drosophila Proteins, Insect Genes, Mesoderm, Nuclear Proteins, Phosphoproteins, Signal Transduction, Transcription Factors, Ubiquitin-Protein Ligases, Zygote, Animals, Drosophila
Source:Developmental Cell
ISSN:1534-5807
Publisher:Cell Press
Volume:10
Number:2
Page Range:257-264
Date:February 2006
Official Publication:https://doi.org/10.1016/j.devcel.2006.01.011
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library