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A Y2H-seq approach defines the human protein methyltransferase interactome

Item Type:Article
Title:A Y2H-seq approach defines the human protein methyltransferase interactome
Creators Name:Weimann, M. and Grossmann, A. and Woodsmith, J. and Özkan, Z. and Birth, P. and Meierhofer, D. and Benlasfer, N. and Valovka, T. and Timmermann, B. and Wanker, E.E. and Sauer, S. and Stelzl, U.
Abstract:To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.
Keywords:DNA Sequence Analysis, Escherichia coli, Enzymologic Gene Expression Regulation, HEK293 Cells, Liquid Chromatography, Methyltransferases, Protein Interaction Mapping, Sensitivity and Specificity, Tandem Mass Spectrometry, Two-Hybrid System Techniques
Source:Nature Methods
Publisher:Nature Publishing Group
Page Range:339-342
Date:April 2013
Official Publication:https://doi.org/10.1038/nmeth.2397
PubMed:View item in PubMed

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