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Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila

Item Type:Article
Title:Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila
Creators Name:Chekulaeva, M. and Filipowicz, W. and Parker, R.
Abstract:miRNA-mediated repression affects a wide range of biological processes including development and human pathologies. The GW182 protein is a key component of miRNA repression complex, recruited by Argonaute and functioning downstream to repress translation and accelerate mRNA degradation, but little is known about how GW182 proteins act. Using both tethered function and complementation assays, we identify three independent domains of the Drosophila GW182 protein (also termed Gawky) that are sufficient to repress mRNA. Each of these domains also functions independently of poly(A) tails. These results indicate that miRNA-mediated repression is facilitated by multiple domains of GW182.
Keywords:GW182, miRNA, mRNA, Translational Control, Animals, Drosophila melanogaster
Source:RNA
ISSN:1355-8382
Publisher:Cold Spring Harbor Lab Press (U.S.A.)
Volume:15
Number:5
Page Range:794-803
Date:May 2009
Official Publication:https://doi.org/10.1261/rna.1364909
PubMed:View item in PubMed

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