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Hierarchic stochastic modelling applied to intracellular Ca(2+) signals

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Item Type:Article
Title:Hierarchic stochastic modelling applied to intracellular Ca(2+) signals
Creators Name:Moenke, G. and Falcke, M. and Thurley, K.
Abstract:Important biological processes like cell signalling and gene expression have noisy components and are very complex at the same time. Mathematical analysis of such systems has often been limited to the study of isolated subsystems, or approximations are used that are difficult to justify. Here we extend a recently published method (Thurley and Falcke, PNAS 2011) which is formulated in observable system configurations instead of molecular transitions. This reduces the number of system states by several orders of magnitude and avoids fitting of kinetic parameters. The method is applied to [Formula: see text] signalling. [Formula: see text] is a ubiquitous second messenger transmitting information by stochastic sequences of concentration spikes, which arise by coupling of subcellular [Formula: see text] release events (puffs). We derive analytical expressions for a mechanistic [Formula: see text] model, based on recent data from live cell imaging, and calculate [Formula: see text] spike statistics in dependence on cellular parameters like stimulus strength or number of [Formula: see text] channels. The new approach substantiates a generic [Formula: see text] model, which is a very convenient way to simulate [Formula: see text] spike sequences with correct spiking statistics.
Keywords:Calcium, Calcium Signaling, Cell Physiological Phenomena, Inositol 1,4,5-Trisphosphate Receptors, Mathematics, Theoretical Models
Source:PLoS ONE
Publisher:Public Library of Science
Page Range:e51178
Date:27 December 2012
Additional Information:Erratum in: PLoS ONE 8(6): 2013.
Official Publication:https://doi.org/10.1371/journal.pone.0051178
PubMed:View item in PubMed

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