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Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis

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Item Type:Article
Title:Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis
Creators Name:Dal Santo, S. and Stampfl, H. and Krasensky, J. and Kempa, S. and Gibon, Y. and Petutschnig, E. and Rozhon, W. and Heuck, A. and Clausen, T. and Jonak, C.
Abstract:Diverse stresses such as high salt conditions cause an increase in reactive oxygen species (ROS), necessitating a redox stress response. However, little is known about the signaling pathways that regulate the antioxidant system to counteract oxidative stress. Here, we show that a Glycogen Synthase Kinase3 from Arabidopsis thaliana (ASKα) regulates stress tolerance by activating Glc-6-phosphate dehydrogenase (G6PD), which is essential for maintaining the cellular redox balance. Loss of stress-activated ASKα leads to reduced G6PD activity, elevated levels of ROS, and enhanced sensitivity to salt stress. Conversely, plants overexpressing ASKα have increased G6PD activity and low levels of ROS in response to stress and are more tolerant to salt stress. ASKα stimulates the activity of a specific cytosolic G6PD isoform by phosphorylating the evolutionarily conserved Thr-467, which is implicated in cosubstrate binding. Our results reveal a novel mechanism of G6PD adaptive regulation that is critical for the cellular stress response.
Keywords:Arabidopsis, Arabidopsis Proteins, Binding Sites, Cell Culture Techniques, Culture Media, Enzyme Activation, Enzymologic Gene Expression Regulation, Genetically Modified Plants, Genetically Modified Plants, Germination, Glycogen Synthase Kinase 3, Glucosephosphate Dehydrogenas, Isoenzymes, Plant Gene Expression Regulation, Oxidation-Reduction, Oxidative Stress, Phosphorylation, Reactive Oxygen Species, Salt-Tolerant Plants, Signal Transduction, Sodium Chloride, Threonine
Source:Plant Cell
Publisher:American Society of Plant Biologists
Page Range:3380-3392
Date:August 2012
Official Publication:https://doi.org/10.1105/tpc.112.101279
PubMed:View item in PubMed

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