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Creating nanoscopic collagen matrices using atomic force microscopy

Item Type:Article
Title:Creating nanoscopic collagen matrices using atomic force microscopy
Creators Name:Jiang, F. and Khairy, K. and Poole, K. and Howard, J. and Mueller, D.J.
Abstract:The atomic force microscope (AFM) is introduced as a biomolecular manipulation machine capable of assembling biological molecules into well-defined molecular structures. Native collagen molecules were mechanically directed into well-defined, two-dimensional templates exhibiting patterns with feature sizes ranging from a few nanometers to several hundreds of micrometers. The resulting nanostructured collagen matrices were only approximately 3-nm thick, exhibited an extreme mechanical stability, and maintained their properties over the time range of several months. Our results directly demonstrate the plasticity of biological assemblies and provide insight into the physical mechanisms by which biological structures may be organized by cells in vivo. These nanoscopic templates may serve as platforms on non-biological surfaces to direct molecular and cellular processes.
Keywords:Atomic Force Microscopy, Collagen Type I, Molecular Interactions, Protein Assembly, Microfibrils, Animals
Source:Microscopy Research and Technique
Page Range:435-440
Date:August 2004
Official Publication:https://doi.org/10.1002/jemt.20101
PubMed:View item in PubMed

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