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The protein expression landscape of the Arabidopsis root

Item Type:Article
Title:The protein expression landscape of the Arabidopsis root
Creators Name:Petricka, J.J. and Schauer, M.A. and Megraw, M. and Breakfield, N.W. and Thompson, J.W. and Georgiev, S. and Soderblom, E.J. and Ohler, U. and Moseley, M.A. and Grossniklaus, U. and Benfey, P.N.
Abstract:Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.
Keywords:Cell-Type Expression, FACS, Plant Proteome, RNA-Protein Correlation, Root Hair Mutant
Source:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
Page Range:6811-6818
Date:1 May 2012
Official Publication:https://doi.org/10.1073/pnas.1202546109
PubMed:View item in PubMed

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