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CMV-specific central memory T cells reside in bone marrow

Item Type:Article
Title:CMV-specific central memory T cells reside in bone marrow
Creators Name:Letsch, A. and Knoedler, M. and Na, I.K. and Kern, F. and Asemissen, A.M. and Keilholz, U. and Loesch, M. and Thiel, E. and Volk, H.D. and Scheibenbogen, C.
Abstract:CMV-specific CD8(+) T cell responses in peripheral blood (PB) are characterized by a preponderance of effector and effector memory T cells. CMV-specific central memory T cells (T(CM)), which are considered crucial in maintaining long-term immunity, are rarely detectable in PB. In this study we have analyzed differentiation and function of CMV pp65-specific CD8(+) T cells in paired samples of human PB and BM using intracellular cytokine and tetramer staining. Overall frequencies of CMV pp65-specific T cells were similar in PB compared to BM; however, CMV-specific CD45RA(-)CCR7(+) T(CM) were almost exclusively detectable in BM, which was not related to a general accumulation of T(CM) in BM. In vitro, CMV-specific T cells could be more efficiently expanded from BM (median 128-fold, n=6) than from PB (median 72-fold, p=0.01). Taken together, these data show that the BM is a compartment harboring CMV-specific T(CM) and underline the concept of the BM as a secondary immune organ. CMV specific BM-derived T(CM) might be a valuable source for generating T cells for adoptive transfer.
Keywords:Bone Marrow, Central Memory, CMV, T Cells
Source:European Journal of Immunology
Publisher:Wiley (U.S.A.)
Page Range:3063-3068
Date:November 2007
Official Publication:https://doi.org/10.1002/eji.200636930
PubMed:View item in PubMed

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