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Ceacam1 separates graft-versus-host-disease from graft-versus-tumor activity after experimental allogeneic bone marrow transplantation

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Item Type:Article
Title:Ceacam1 separates graft-versus-host-disease from graft-versus-tumor activity after experimental allogeneic bone marrow transplantation
Creators Name:Lu, S.X. and Kappel, L.W. and Charbonneau-Allard, A.M. and Atallah, R. and Holland, A.M. and Turbide, C. and Hubbard, V.M. and Rotolo, J.A. and Smith, M. and Suh, D. and King, C. and Rao, U.K. and Yim, N. and Bautista, J.L. and Jenq, R.R. and Penack, O. and Na, I.K. and Liu, C. and Murphy, G. and Alpdogan, O. and Blumberg, R.S. and Macian, F. and Holmes, K.V. and Beauchemin, N. and van den Brink, M.R.M.
Abstract:Background: Allogeneic bone marrow transplantation (allo-BMT) is a potentially curative therapy for a variety of hematologic diseases, but benefits, including graft-versus-tumor (GVT) activity are limited by graft-versus-host-disease (GVHD). Carcinoembryonic antigen related cell adhesion molecule 1 (Ceacam1) is a transmembrane glycoprotein found on epithelium, T cells, and many tumors. It regulates a variety of physiologic and pathological processes such as tumor biology, leukocyte activation, and energy homeostasis. Previous studies suggest that Ceacam1 negatively regulates inflammation in inflammatory bowel disease models. Methods: We studied Ceacam1 as a regulator of GVHD and GVT after allogeneic bone marrow transplantation (allo-BMT) in mouse models. In vivo, Ceacam1(-/-) T cells caused increased GVHD mortality and GVHD of the colon, and greater numbers of donor T cells were positive for activation markers (CD25(hi), CD62L(lo)). Additionally, Ceacam1(-/-) CD8 T cells had greater expression of the gut-trafficking integrin α(4)β(7), though both CD4 and CD8 T cells were found increased numbers in the gut post-transplant. Ceacam1(-/-) recipients also experienced increased GVHD mortality and GVHD of the colon, and alloreactive T cells displayed increased activation. Additionally, Ceacam1(-/-) mice had increased mortality and decreased numbers of regenerating small intestinal crypts upon radiation exposure. Conversely, Ceacam1-overexpressing T cells caused attenuated target-organ and systemic GVHD, which correlated with decreased donor T cell numbers in target tissues, and mortality. Finally, graft-versus-tumor survival in a Ceacam1(+) lymphoma model was improved in animals receiving Ceacam1(-/-) vs. control T cells. Conclusions: We conclude that Ceacam1 regulates T cell activation, GVHD target organ damage, and numbers of donor T cells in lymphoid organs and GVHD target tissues. In recipients of allo-BMT, Ceacam1 may also regulate tissue radiosensitivity. Because of its expression on both the donor graft and host tissues, this suggests that targeting Ceacam1 may represent a potent strategy for the regulation of GVHD and GVT after allogeneic transplantation.
Keywords:Bone Marrow Transplantation, CD8-Positive T-Lymphocytes, Carcinoembryonic Antigen, Cell Polarity, Cell Proliferation, Dendritic Cells, Experimental Radiation Injuries, Graft vs Host Disease, Graft vs Tumor Effect, Homologous Transplantation, Immunologic Cytotoxicity, Integrins, Ionizing Radiation, Lymphocyte Activation, Lymphocyte Count, Lymphoid Tissue, Organ Specificity, Small Intestine, Animals, Mice
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:6
Number:7
Page Range:e21611
Date:6 July 2011
Official Publication:https://doi.org/10.1371/journal.pone.0021611
PubMed:View item in PubMed

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