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Regulation of ASIC channels by a stomatin/STOML3 complex located in a mobile vesicle pool in sensory neurons

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Item Type:Article
Title:Regulation of ASIC channels by a stomatin/STOML3 complex located in a mobile vesicle pool in sensory neurons
Creators Name:Lapatsina, L. and Jira, J.A. and Smith, E.S.J. and Poole, K. and Kozlenkov, A. and Bilbao, D. and Lewin, G.R. and Heppenstall, P.A.
Abstract:A complex of stomatin-family proteins and acid-sensing (proton-gated) ion channel (ASIC) family members participate in sensory transduction in invertebrates and vertebrates. Here, we have examined the role of the stomatin-family protein stomatin-like protein-3 (STOML3) in this process. We demonstrate that STOML3 interacts with stomatin and ASIC subunits and that this occurs in a highly mobile vesicle pool in dorsal root ganglia (DRG) neurons and Chinese hamster ovary cells. We identify a hydrophobic region in the N-terminus of STOML3 that is required for vesicular localization of STOML3 and regulates physical and functional interaction with ASICs. We further characterize STOML3-containing vesicles in DRG neurons and show that they are Rab11-positive, but not part of the early-endosomal, lysosomal or Rab14-dependent biosynthetic compartment. Moreover, uncoupling of vesicles from microtubules leads to incorporation of STOML3 into the plasma membrane and increased acid-gated currents. Thus, STOML3 defines a vesicle pool in which it associates with molecules that have critical roles in sensory transduction. We suggest that the molecular features of this vesicular pool may be characteristic of a 'transducosome' in sensory neurons.
Keywords:Ion Channel, Mechanotransduction, Peripheral Sensory Neurons, Stomatin-Like Proteins, Animals, Mice
Source:Open Biology
Publisher:Royal Society
Page Range:120096
Date:27 June 2012
Official Publication:https://doi.org/10.1098/rsob.120096
PubMed:View item in PubMed

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