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In vivo quantitative proteome profiling: planning and evaluation of SILAC experiments

Item Type:Article
Title:In vivo quantitative proteome profiling: planning and evaluation of SILAC experiments
Creators Name:Kirchner, M. and Selbach, M.
Abstract:Mass spectrometry-based quantitative proteomics can identify and quantify thousands of proteins in complex biological samples. Improved instrumentation, quantification strategies and data analysis tools now enable protein analysis on a genome-wide scale. Particularly, quantification based on stable isotope labeling with amino acids (SILAC) has emerged as a robust, reliable and simple method for accurate large-scale protein quantification. The spectrum of applications ranges from bacteria and eukaryotic cell culture systems to multicellular organisms. Here, we provide a step-by-step protocol on how to plan and perform large-scale quantitative proteome analysis using SILAC, from sample preparation to final data analysis.
Keywords:In Vivo SILAC, Animal Model, MaxQuant, Large-Scale Proteomics, Quantitative Mass Spectrometry, Animals
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Quantative methods in proteomics
ISSN:1064-3745
ISBN:978-1-61779-884-9
Publisher:Springer / Humana Press (U.S.A.)
Volume:893
Page Range:175-199
Number of Pages:539
Date:7 May 2012
Official Publication:https://doi.org/10.1007/978-1-61779-885-6_13
PubMed:View item in PubMed

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