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Gene expression of pluripotency determinants is conserved between mammalian and planarian stem cells

Item Type:Article
Title:Gene expression of pluripotency determinants is conserved between mammalian and planarian stem cells
Creators Name:Onal, P. and Gruen, D. and Adamidi, C. and Rybak, A. and Solana, J. and Mastrobuoni, G. and Wang, Y. and Rahn, H.P. and Chen, W. and Kempa, S. and Ziebold, U. and Rajewsky, N.
Abstract:Freshwater planaria possess extreme regeneration capabilities mediated by abundant, pluripotent stem cells (neoblasts) in adult animals. Although planaria emerged as an attractive in vivo model system for stem cell biology, gene expression in neoblasts has not been profiled comprehensively and it is unknown how molecular mechanisms for pluripotency in neoblasts relate to those in mammalian embryonic stem cells (ESCs). We purified neoblasts and quantified mRNA and protein expression by sequencing and shotgun proteomics. We identified ∼4000 genes specifically expressed in neoblasts, including all ∼30 known neoblast markers. Genes important for pluripotency in ESCs, including regulators as well as targets of OCT4, were well conserved and upregulated in neoblasts. We found conserved expression of epigenetic regulators and demonstrated their requirement for planarian regeneration by knockdown experiments. Post-transcriptional regulatory genes characteristic for germ cells were also enriched in neoblasts, suggesting the existence of a common ancestral state of germ cells and ESCs. We conclude that molecular determinants of pluripotency are conserved throughout evolution and that planaria are an informative model system for human stem cell biology.
Keywords:Epigenetic Regulators, Neoblasts, Planaria, Pluripotency, Stem Cells, Animals
Source:EMBO Journal
Publisher:Nature Publishing Group
Page Range:2755-2769
Date:27 April 2012
Official Publication:https://doi.org/10.1038/emboj.2012.110
PubMed:View item in PubMed

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