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Retargeting transposon insertions by the adeno-associated virus Rep protein

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Item Type:Article
Title:Retargeting transposon insertions by the adeno-associated virus Rep protein
Creators Name:Ammar, I. and Gogol-Doering, A. and Miskey, C. and Chen, W. and Cathomen, T. and Izsvak, Z. and Ivics, Z.
Abstract:The Sleeping Beauty (SB), piggyBac (PB) and Tol2 transposons are promising instruments for genome engineering. Integration site profiling of SB, PB and Tol2 in human cells showed that PB and Tol2 insertions were enriched in genes, whereas SB insertions were randomly distributed. We aimed to introduce a bias into the target site selection properties of the transposon systems by taking advantage of the locus-specific integration system of adeno-associated virus (AAV). The AAV Rep protein binds to Rep recognition sequences (RRSs) in the human genome, and mediates viral integration into nearby sites. A series of fusion constructs consisting of the N-terminal DNA-binding domain of Rep and the transposases or the N57 domain of SB were generated. A plasmid-based transposition assay showed that Rep/SB yielded a 15-fold enrichment of transposition at a particular site near a targeted RRS. Genome-wide insertion site analysis indicated that an approach based on interactions between the SB transposase and Rep/N57 enriched transgene insertions at RRSs. We also provide evidence of biased insertion of the PB and Tol2 transposons. This study provides a comparative insight into target site selection properties of transposons, as well as proof-of-principle for targeted chromosomal transposition by composite protein-protein and protein-DNA interactions.
Keywords:Binding Sites, Dependovirus, DNA, DNA-Binding Proteins, DNA Transposable Elements, HeLa Cells, Plasmids, Protein Interaction Domains and Motifs, Recombinant Fusion Proteins, Transposases, Viral Proteins, Virus Integration
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:6693-6712
Date:1 August 2012
Official Publication:https://doi.org/10.1093/nar/gks317
PubMed:View item in PubMed

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