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In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans

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Item Type:Article
Title:In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans
Creators Name:Ma, L. and Tan, Z. and Teng, Y. and Hoersch, S. and Horvitz, H.R.
Abstract:The in vivo analysis of the roles of splicing factors in regulating alternative splicing in animals remains a challenge. Using a microarray-based screen, we identified a Caenorhabditis elegans gene, tos-1, that exhibited three of the four major types of alternative splicing: intron retention, exon skipping, and, in the presence of U2AF large subunit mutations, the use of alternative 3' splice sites. Mutations in the splicing factors U2AF large subunit and SF1/BBP altered the splicing of tos-1. 3' splice sites of the retained intron or before the skipped exon regulate the splicing pattern of tos-1. Our study provides in vivo evidence that intron retention and exon skipping can be regulated largely by the identities of 3' splice sites.
Keywords:C. elegans, SFA-1, UAF-1, Alternative Splicing, Animals, Caenorhabditis elegans
Source:RNA
ISSN:1355-8382
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Volume:17
Number:12
Page Range:2201-2211
Date:December 2011
Official Publication:https://doi.org/10.1261/rna.027458.111
PubMed:View item in PubMed

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