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| Item Type: | Article |
|---|---|
| Title: | In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans |
| Creators Name: | Ma, L. and Tan, Z. and Teng, Y. and Hoersch, S. and Horvitz, H.R. |
| Abstract: | The in vivo analysis of the roles of splicing factors in regulating alternative splicing in animals remains a challenge. Using a microarray-based screen, we identified a Caenorhabditis elegans gene, tos-1, that exhibited three of the four major types of alternative splicing: intron retention, exon skipping, and, in the presence of U2AF large subunit mutations, the use of alternative 3' splice sites. Mutations in the splicing factors U2AF large subunit and SF1/BBP altered the splicing of tos-1. 3' splice sites of the retained intron or before the skipped exon regulate the splicing pattern of tos-1. Our study provides in vivo evidence that intron retention and exon skipping can be regulated largely by the identities of 3' splice sites. |
| Keywords: | C. elegans, SFA-1, UAF-1, Alternative Splicing, Animals, Caenorhabditis elegans |
| Source: | RNA |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Volume: | 17 |
| Number: | 12 |
| Page Range: | 2201-2211 |
| Date: | December 2011 |
| Official Publication: | https://doi.org/10.1261/rna.027458.111 |
| PubMed: | View item in PubMed |
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